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rnascope probe m. musculus wnt2  (Advanced Cell Diagnostics Inc)

 
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    Structured Review

    Advanced Cell Diagnostics Inc rnascope probe m. musculus wnt2
    ( A ) Immunofluorescence staining against GFP (green) and SOX2 (magenta) in Axin2 CreERT2/+ ; ROSA26 mTmG/+ pituitaries 48 hr post-induction. Graph representing a quantification of the proximity of individual GFP + cells to the nearest SOX2 + cell as quantified by the number of nuclei separating them. Plotted data represents the proportion of GFP+ cells that fall into each category of the total GFP+ cells, taken from n = 3 separate pituitaries. Scale bars: 50 μm. ( B ) Experimental paradigm for RNA Seq analysis of Sox2 positive and negative cells. ( C ) Graphs representing the FPKM values of Wls and Porcupine in Sox2 positive and negative cells (black and grey bars, respectively). mRNA in situ hybridisation for Sox2 and for Wls on wild-type sagittal pituitaries at P14, demonstrating strong Wls expression in the marginal zone epithelium. Scale bars: 250 μm. ( D ) Bar chart showing the FPKM values of Wnt genes in the Sox2 + and Sox2 − fractions. Double mRNA in situ hybridisation against <t>Wnt2</t> , Wnt5a, and Wnt9a (blue) together with Sox2 (red) validating expression in the Sox2 + population. Boxed regions through the marginal zone epithelium are magnified. Scale bars: 100 μm and 50 μm in boxed inserts.
    Rnascope Probe M. Musculus Wnt2, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+probe+m%2E+musculus+wnt2/pmc07803373-47-3-9?v=Advanced+Cell+Diagnostics+Inc
    Average 90 stars, based on 1 article reviews
    rnascope probe m. musculus wnt2 - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells"

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells

    Journal: eLife

    doi: 10.7554/eLife.59142

    ( A ) Immunofluorescence staining against GFP (green) and SOX2 (magenta) in Axin2 CreERT2/+ ; ROSA26 mTmG/+ pituitaries 48 hr post-induction. Graph representing a quantification of the proximity of individual GFP + cells to the nearest SOX2 + cell as quantified by the number of nuclei separating them. Plotted data represents the proportion of GFP+ cells that fall into each category of the total GFP+ cells, taken from n = 3 separate pituitaries. Scale bars: 50 μm. ( B ) Experimental paradigm for RNA Seq analysis of Sox2 positive and negative cells. ( C ) Graphs representing the FPKM values of Wls and Porcupine in Sox2 positive and negative cells (black and grey bars, respectively). mRNA in situ hybridisation for Sox2 and for Wls on wild-type sagittal pituitaries at P14, demonstrating strong Wls expression in the marginal zone epithelium. Scale bars: 250 μm. ( D ) Bar chart showing the FPKM values of Wnt genes in the Sox2 + and Sox2 − fractions. Double mRNA in situ hybridisation against Wnt2 , Wnt5a, and Wnt9a (blue) together with Sox2 (red) validating expression in the Sox2 + population. Boxed regions through the marginal zone epithelium are magnified. Scale bars: 100 μm and 50 μm in boxed inserts.
    Figure Legend Snippet: ( A ) Immunofluorescence staining against GFP (green) and SOX2 (magenta) in Axin2 CreERT2/+ ; ROSA26 mTmG/+ pituitaries 48 hr post-induction. Graph representing a quantification of the proximity of individual GFP + cells to the nearest SOX2 + cell as quantified by the number of nuclei separating them. Plotted data represents the proportion of GFP+ cells that fall into each category of the total GFP+ cells, taken from n = 3 separate pituitaries. Scale bars: 50 μm. ( B ) Experimental paradigm for RNA Seq analysis of Sox2 positive and negative cells. ( C ) Graphs representing the FPKM values of Wls and Porcupine in Sox2 positive and negative cells (black and grey bars, respectively). mRNA in situ hybridisation for Sox2 and for Wls on wild-type sagittal pituitaries at P14, demonstrating strong Wls expression in the marginal zone epithelium. Scale bars: 250 μm. ( D ) Bar chart showing the FPKM values of Wnt genes in the Sox2 + and Sox2 − fractions. Double mRNA in situ hybridisation against Wnt2 , Wnt5a, and Wnt9a (blue) together with Sox2 (red) validating expression in the Sox2 + population. Boxed regions through the marginal zone epithelium are magnified. Scale bars: 100 μm and 50 μm in boxed inserts.

    Techniques Used: Immunofluorescence, Staining, RNA Sequencing, In Situ, Hybridization, Expressing


    Figure Legend Snippet:

    Techniques Used: Isolation, Sequencing, RNAscope, Positive Control, Negative Control, Recombinant, Staining, Software



    Similar Products

    rnascope probe m. musculus wnt2  (Advanced Cell Diagnostics Inc)
    90
    Advanced Cell Diagnostics Inc rnascope probe m. musculus wnt2
    ( A ) Immunofluorescence staining against GFP (green) and SOX2 (magenta) in Axin2 CreERT2/+ ; ROSA26 mTmG/+ pituitaries 48 hr post-induction. Graph representing a quantification of the proximity of individual GFP + cells to the nearest SOX2 + cell as quantified by the number of nuclei separating them. Plotted data represents the proportion of GFP+ cells that fall into each category of the total GFP+ cells, taken from n = 3 separate pituitaries. Scale bars: 50 μm. ( B ) Experimental paradigm for RNA Seq analysis of Sox2 positive and negative cells. ( C ) Graphs representing the FPKM values of Wls and Porcupine in Sox2 positive and negative cells (black and grey bars, respectively). mRNA in situ hybridisation for Sox2 and for Wls on wild-type sagittal pituitaries at P14, demonstrating strong Wls expression in the marginal zone epithelium. Scale bars: 250 μm. ( D ) Bar chart showing the FPKM values of Wnt genes in the Sox2 + and Sox2 − fractions. Double mRNA in situ hybridisation against <t>Wnt2</t> , Wnt5a, and Wnt9a (blue) together with Sox2 (red) validating expression in the Sox2 + population. Boxed regions through the marginal zone epithelium are magnified. Scale bars: 100 μm and 50 μm in boxed inserts.
    Rnascope Probe M. Musculus Wnt2, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+probe+m%2E+musculus+wnt2/pmc07803373-47-3-9?v=Advanced+Cell+Diagnostics+Inc
    Average 90 stars, based on 1 article reviews
    rnascope probe m. musculus wnt2 - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Immunofluorescence staining against GFP (green) and SOX2 (magenta) in Axin2 CreERT2/+ ; ROSA26 mTmG/+ pituitaries 48 hr post-induction. Graph representing a quantification of the proximity of individual GFP + cells to the nearest SOX2 + cell as quantified by the number of nuclei separating them. Plotted data represents the proportion of GFP+ cells that fall into each category of the total GFP+ cells, taken from n = 3 separate pituitaries. Scale bars: 50 μm. ( B ) Experimental paradigm for RNA Seq analysis of Sox2 positive and negative cells. ( C ) Graphs representing the FPKM values of Wls and Porcupine in Sox2 positive and negative cells (black and grey bars, respectively). mRNA in situ hybridisation for Sox2 and for Wls on wild-type sagittal pituitaries at P14, demonstrating strong Wls expression in the marginal zone epithelium. Scale bars: 250 μm. ( D ) Bar chart showing the FPKM values of Wnt genes in the Sox2 + and Sox2 − fractions. Double mRNA in situ hybridisation against Wnt2 , Wnt5a, and Wnt9a (blue) together with Sox2 (red) validating expression in the Sox2 + population. Boxed regions through the marginal zone epithelium are magnified. Scale bars: 100 μm and 50 μm in boxed inserts.

    Journal: eLife

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells

    doi: 10.7554/eLife.59142

    Figure Lengend Snippet: ( A ) Immunofluorescence staining against GFP (green) and SOX2 (magenta) in Axin2 CreERT2/+ ; ROSA26 mTmG/+ pituitaries 48 hr post-induction. Graph representing a quantification of the proximity of individual GFP + cells to the nearest SOX2 + cell as quantified by the number of nuclei separating them. Plotted data represents the proportion of GFP+ cells that fall into each category of the total GFP+ cells, taken from n = 3 separate pituitaries. Scale bars: 50 μm. ( B ) Experimental paradigm for RNA Seq analysis of Sox2 positive and negative cells. ( C ) Graphs representing the FPKM values of Wls and Porcupine in Sox2 positive and negative cells (black and grey bars, respectively). mRNA in situ hybridisation for Sox2 and for Wls on wild-type sagittal pituitaries at P14, demonstrating strong Wls expression in the marginal zone epithelium. Scale bars: 250 μm. ( D ) Bar chart showing the FPKM values of Wnt genes in the Sox2 + and Sox2 − fractions. Double mRNA in situ hybridisation against Wnt2 , Wnt5a, and Wnt9a (blue) together with Sox2 (red) validating expression in the Sox2 + population. Boxed regions through the marginal zone epithelium are magnified. Scale bars: 100 μm and 50 μm in boxed inserts.

    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Wnt2 , Advanced Cell Diagnostics , 313601 , .

    Techniques: Immunofluorescence, Staining, RNA Sequencing, In Situ, Hybridization, Expressing

    Journal: eLife

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells

    doi: 10.7554/eLife.59142

    Figure Lengend Snippet:

    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Wnt2 , Advanced Cell Diagnostics , 313601 , .

    Techniques: Isolation, Sequencing, RNAscope, Positive Control, Negative Control, Recombinant, Staining, Software